WEKO3
アイテム
{"_buckets": {"deposit": "9353f11e-988c-42a9-b711-f3749821276f"}, "_deposit": {"created_by": 14, "id": "5405", "owners": [14], "pid": {"revision_id": 0, "type": "depid", "value": "5405"}, "status": "published"}, "_oai": {"id": "oai:kpu.repo.nii.ac.jp:00005405", "sets": ["456"]}, "author_link": ["9960", "9959"], "control_number": "5405", "item_1696926521561": {"attribute_name": "その他(別言語等)", "attribute_value_mlt": [{"subitem_alternative_title": "Characteristics of Polyphenol Oxidase in Loquat (B. LIVING SCIENCE)", "subitem_alternative_title_language": "en"}]}, "item_3_biblio_info_12": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1985-11-15", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "29", "bibliographicPageStart": "25", "bibliographicVolumeNumber": "36", "bibliographic_titles": [{"bibliographic_title": "京都府立大学学術報告. 理学・生活科学", "bibliographic_titleLang": "ja"}, {"bibliographic_title": "The scientific reports of the Kyoto Prefectural University. Natural science and living science", "bibliographic_titleLang": "en"}]}]}, "item_3_description_10": {"attribute_name": "抄録(日)", "attribute_value_mlt": [{"subitem_description": "びわ果実アセトンパウダーよりポリフェノールオキシダーゼを抽出し, 30\u0026acd;90%硫安分画にて酵素を部分精製した。本酵素の等電点は, 焦点電気泳動よりpH 3.5,また分子量はSephadex G-100のゲル濾過より42,000と推定した。本酵素はο-ジヒドロキシフェノールに対して広い基質特異性を示し, その至適pHは6.8(ピロカテコール, D-カテキン)と5.9(4-メチルカテコール)であった。熱に対しては, 本酵素は60℃, 30分間処理ではほとんど失活せず, 80℃, 30分間処理でほぼ失活した。また, pH 4\u0026acd;6の弱酸性域で最も安定であった。", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_3_description_11": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "A polyphenol oxidase was extracted from acetone powder of loquat with 0.1M Naphosphate buffer, pH 6.5 and partially purified by 30\u0026acd;90% ammonium sulfate fractionation. Isoelectric focusing and Sephadex G-100gel filtration of the preparation indicated that the enzyme had an isoelectric point of 3.5 and a molecular weight of 42,000. The enzyme showed wide substrate specificity toward ο-dihydroxyphenols. Optimum pHs were 6.8 when pyrocatechol and D-catechin were used as substrate and 5.9 in case of 4-methylcatechol as substrate. The enzyme was stable at 60℃ for 30 min but it lost more than 95% of the activity after 30min at 80℃. The enzyme maintained almost its whole activity for 24 hr at 25℃ in the pH range from 4 to 6.", "subitem_description_language": "en", "subitem_description_type": "Other"}]}, "item_3_source_id_1": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00062300", "subitem_source_identifier_type": "NCID"}]}, "item_3_source_id_20": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0075739X", "subitem_source_identifier_type": "PISSN"}]}, "item_3_text_6": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_language": "ja", "subitem_text_value": "京都府立大学食物学科栄養学講座"}, {"subitem_text_language": "ja", "subitem_text_value": "京都府立大学食物学科栄養学講座"}]}, "item_3_text_7": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Laboratory of Nutrition, Department of Food Science, Kyoto Prefectural University"}, {"subitem_text_language": "en", "subitem_text_value": "Laboratory of Nutrition, Department of Food Science, Kyoto Prefectural University"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorAffiliations": [{"affiliationNameIdentifiers": [], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "牧, 善輔", "creatorNameLang": "ja"}, {"creatorName": "マキ, ゼンスケ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Maki, Zensuke", "creatorNameLang": "en"}], "familyNames": [{"familyName": "牧", "familyNameLang": "ja"}, {"familyName": "マキ", "familyNameLang": "ja-Kana"}, {"familyName": "Maki", "familyNameLang": "en"}], "givenNames": [{"givenName": "善輔", "givenNameLang": "ja"}, {"givenName": "ゼンスケ", "givenNameLang": "ja-Kana"}, {"givenName": "Zensuke", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9960", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "田代, 操", "creatorNameLang": "ja"}, {"creatorName": "タシロ, ミサオ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Tashiro, Misao", "creatorNameLang": "en"}], "familyNames": [{"familyName": "田代", "familyNameLang": "ja"}, {"familyName": "タシロ", "familyNameLang": "ja-Kana"}, {"familyName": "Tashiro", "familyNameLang": "en"}], "givenNames": [{"givenName": "操", "givenNameLang": "ja"}, {"givenName": "ミサオ", "givenNameLang": "ja-Kana"}, {"givenName": "Misao", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9959", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-02-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00000079531.pdf", "filesize": [{"value": "321.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "application/pdf", "size": 321200.0, "url": {"label": "KJ00000079531.pdf", "url": "https://kpu.repo.nii.ac.jp/record/5405/files/KJ00000079531.pdf"}, "version_id": "206627d2-46c8-4b9f-991c-dadaeec4b648"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "びわ果実ポリフェノールオキシダーゼの性質(B. 生活科学)", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "びわ果実ポリフェノールオキシダーゼの性質(B. 生活科学)", "subitem_title_language": "ja"}]}, "item_type_id": "3", "owner": "14", "path": ["456"], "permalink_uri": "https://kpu.repo.nii.ac.jp/records/5405", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-02-20"}, "publish_date": "2017-02-20", "publish_status": "0", "recid": "5405", "relation": {}, "relation_version_is_last": true, "title": ["びわ果実ポリフェノールオキシダーゼの性質(B. 生活科学)"], "weko_shared_id": -1}
びわ果実ポリフェノールオキシダーゼの性質(B. 生活科学)
https://kpu.repo.nii.ac.jp/records/5405
https://kpu.repo.nii.ac.jp/records/5405a25c331f-d8c8-4662-9d2d-f6d531fa6065
名前 / ファイル | ライセンス | アクション |
---|---|---|
KJ00000079531.pdf (321.2 kB)
|
|
Item type | [ELS]紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2017-02-20 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | びわ果実ポリフェノールオキシダーゼの性質(B. 生活科学) | |||||
その他(別言語等) | ||||||
その他のタイトル | Characteristics of Polyphenol Oxidase in Loquat (B. LIVING SCIENCE) | |||||
言語 | en | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
牧, 善輔
× 牧, 善輔× 田代, 操 |
|||||
抄録(日) | ||||||
内容記述タイプ | Other | |||||
内容記述 | びわ果実アセトンパウダーよりポリフェノールオキシダーゼを抽出し, 30∿90%硫安分画にて酵素を部分精製した。本酵素の等電点は, 焦点電気泳動よりpH 3.5,また分子量はSephadex G-100のゲル濾過より42,000と推定した。本酵素はο-ジヒドロキシフェノールに対して広い基質特異性を示し, その至適pHは6.8(ピロカテコール, D-カテキン)と5.9(4-メチルカテコール)であった。熱に対しては, 本酵素は60℃, 30分間処理ではほとんど失活せず, 80℃, 30分間処理でほぼ失活した。また, pH 4∿6の弱酸性域で最も安定であった。 | |||||
言語 | ja | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | A polyphenol oxidase was extracted from acetone powder of loquat with 0.1M Naphosphate buffer, pH 6.5 and partially purified by 30∿90% ammonium sulfate fractionation. Isoelectric focusing and Sephadex G-100gel filtration of the preparation indicated that the enzyme had an isoelectric point of 3.5 and a molecular weight of 42,000. The enzyme showed wide substrate specificity toward ο-dihydroxyphenols. Optimum pHs were 6.8 when pyrocatechol and D-catechin were used as substrate and 5.9 in case of 4-methylcatechol as substrate. The enzyme was stable at 60℃ for 30 min but it lost more than 95% of the activity after 30min at 80℃. The enzyme maintained almost its whole activity for 24 hr at 25℃ in the pH range from 4 to 6. | |||||
言語 | en | |||||
書誌情報 |
ja : 京都府立大学学術報告. 理学・生活科学 en : The scientific reports of the Kyoto Prefectural University. Natural science and living science 巻 36, p. 25-29, 発行日 1985-11-15 |
|||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00062300 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0075739X |