{"created":"2023-06-19T08:46:32.000141+00:00","id":5405,"links":{},"metadata":{"_buckets":{"deposit":"9353f11e-988c-42a9-b711-f3749821276f"},"_deposit":{"created_by":14,"id":"5405","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"5405"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00005405","sets":["47:274:456"]},"author_link":["9960","9959"],"control_number":"5405","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"Characteristics of Polyphenol Oxidase in Loquat (B. LIVING SCIENCE)","subitem_alternative_title_language":"en"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1985-11-15","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"29","bibliographicPageStart":"25","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"京都府立大学学術報告. 理学・生活科学","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of the Kyoto Prefectural University. Natural science and living science","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"びわ果実アセトンパウダーよりポリフェノールオキシダーゼを抽出し, 30∿90%硫安分画にて酵素を部分精製した。本酵素の等電点は, 焦点電気泳動よりpH 3.5,また分子量はSephadex G-100のゲル濾過より42,000と推定した。本酵素はο-ジヒドロキシフェノールに対して広い基質特異性を示し, その至適pHは6.8(ピロカテコール, D-カテキン)と5.9(4-メチルカテコール)であった。熱に対しては, 本酵素は60℃, 30分間処理ではほとんど失活せず, 80℃, 30分間処理でほぼ失活した。また, pH 4∿6の弱酸性域で最も安定であった。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"A polyphenol oxidase was extracted from acetone powder of loquat with 0.1M Naphosphate buffer, pH 6.5 and partially purified by 30∿90% ammonium sulfate fractionation. Isoelectric focusing and Sephadex G-100gel filtration of the preparation indicated that the enzyme had an isoelectric point of 3.5 and a molecular weight of 42,000. The enzyme showed wide substrate specificity toward ο-dihydroxyphenols. Optimum pHs were 6.8 when pyrocatechol and D-catechin were used as substrate and 5.9 in case of 4-methylcatechol as substrate. The enzyme was stable at 60℃ for 30 min but it lost more than 95% of the activity after 30min at 80℃. The enzyme maintained almost its whole activity for 24 hr at 25℃ in the pH range from 4 to 6.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062300","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0075739X","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"牧, 善輔","creatorNameLang":"ja"},{"creatorName":"マキ, ゼンスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Maki, Zensuke","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田代, 操","creatorNameLang":"ja"},{"creatorName":"タシロ, ミサオ","creatorNameLang":"ja-Kana"},{"creatorName":"Tashiro, Misao","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000079531.pdf","filesize":[{"value":"321.2 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000079531.pdf","url":"https://kpu.repo.nii.ac.jp/record/5405/files/KJ00000079531.pdf"},"version_id":"206627d2-46c8-4b9f-991c-dadaeec4b648"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"びわ果実ポリフェノールオキシダーゼの性質(B. 生活科学)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"びわ果実ポリフェノールオキシダーゼの性質(B. 生活科学)","subitem_title_language":"ja"}]},"item_type_id":"3","owner":"14","path":["456"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"5405","relation_version_is_last":true,"title":["びわ果実ポリフェノールオキシダーゼの性質(B. 生活科学)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-02-29T05:14:30.680880+00:00"}