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多量の疫病菌遊走子のうを得るための紙蓋平面培養法(農学部門)
https://kpu.repo.nii.ac.jp/records/4856
https://kpu.repo.nii.ac.jp/records/48567ad67d76-bb68-49a2-a65b-ee19169148dc
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000078855.pdf (457.6 kB)
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| Item type | [ELS]紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-02-20 | |||||
| タイトル | ||||||
| タイトル | 多量の疫病菌遊走子のうを得るための紙蓋平面培養法(農学部門) | |||||
| 言語 | ja | |||||
| その他（別言語等） | ||||||
| その他のタイトル | Paper lid method for cultubation of numerous zoosporangia in Phytophthora capsici (Agriculture) | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
宮田, 善雄
× 宮田, 善雄× 桂, 琦一× 室川, 嗣夫 |
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| 抄録(日) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Phytophthora capsici Leon.の遊走子のうを多量に形成させるための培養法を開拓した。培地としてはキュウリ, ダイコン, タマネギ, ナス, ニンジン, および, キャベツの食用部分のジュースのうちで, キュウリが最もすぐれていた。このジュースは果実をミキサーで粉砕後4枚のガーゼで滬過した程度がよく, 混在する組織断片や細胞顆粒の中に旺盛な遊走子型発芽能力をもった遊走子のうの形成に有効な物質が含れている。このジュースは原液のままより, むしろ, 2倍程度に稀釈したほうが遊走子のうの形成には適している。この培地に菌体を植込み, 28℃で2週間, 前培養したのち, 形成された菌そうを取出して, 滅菌した脱イオン水でよく洗い, 表を上にして, 湿った滬紙を敷いたペトリ皿に移し, 障子紙を張った木枠のふたをかぶせて, 28℃螢火灯照明付定温器内に並べておくと, 24-28時間の後培養で, 菌そうの上面に多量の遊走子のうが形成されるので, これをかき取って供試することになる。 | |||||
| 言語 | ja | |||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Paper lid method for the cultural formation of abundant zoosporangia of Phytophthora capsici Leon. was described on this paper. The juice medium of cucumber was most favorable for obtaining numerous zoosporangia among that of seven vegetable plants, i.e. cucumber, radish, onion, eggplant, carrot, cabbage and V-8 juice (Campbell Soup Co.). Cucumber fruits were homogenized by an electric mixer, and filtered through four sheets of gause. The filtrate contained fine tissue debris and cellular particles, which were indespensable as active factors for production of zoosporangia released vigorously swimming zoospores. The cucumber juice medium was produced by adding same volume of deionized water to the filtrate. Thirty ml of the medium in each of 200ml Erlenmyer flask was sterilized by autoclaving at 121℃ for 15min. Many times of sterilization by autoclaving were unfavorable for mycerial growth and zoosporangium formation. This medium was inoculated with a 5mm diam disk of mycerium grown on PDA plane ager in Petri dish at 28℃ for 4 days. The inoculated medium was incubated at 28℃ for 2 weeks. The grown mycerial mat was removed from the flask, and rinsed with sterilized deionized water through a Buchner funnel with a sheet of 70mm diam filter paper (Toyo No. 2). The clean mycerial mat put on two sheets of 90mm diam wet filter paper in Petri dish which was covered with a special paper lid, instead of ordinary glass lid of Petri dish. The paper lid was a wooden hoop with 100mm diam, 13mm wide and 3mm thick, covered with Japanese paper on upper side. The lid keeps the surface of mycerial mat in moderately dry condition which was very effective for sporangium production by this fungus, and prevents it from being contaminated by another microorganisms. The Petri dish placed in a 28℃ incubator illuminated with fluoresent lamp. The illumination was about 50 ft-c at the surface of the Petri dish. Numerous zoosporangia were formed on the surface of mycerial mat after 24 hours. They were easily collected by a small spatula, or suspended in water by stirring with a short brush. | |||||
| 言語 | en | |||||
| 書誌情報 |
ja : 京都府立大學學術報告. 農學 en : The scientific reports of Kyoto Prefectural University. Agriculture 巻 22, p. 27-30, 発行日 1970-10-15 |
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| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00062275 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 00757373 | |||||
