WEKO3
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抹茶の総アミノ酸および遊離アミノ酸の HPLC-アミノ酸分析機による分析(人間環境学)
https://kpu.repo.nii.ac.jp/records/4208
https://kpu.repo.nii.ac.jp/records/420866b30081-ddc9-41e6-8499-85eb91a2c138
名前 / ファイル | ライセンス | アクション |
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KJ00000041161.pdf (356.3 kB)
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Item type | [ELS]紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-02-20 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 抹茶の総アミノ酸および遊離アミノ酸の HPLC-アミノ酸分析機による分析(人間環境学) | |||||
その他(別言語等) | ||||||
その他のタイトル | Analysis of Total and Free Amino Acids in Mat-cha (Powdered Green Tea) with an HPLC-Amino Acid Analyzer (HUMAN ENVIRONMENT) | |||||
言語 | en | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
大槻, 耕三
× 大槻, 耕三× 奥野, 忍× 谷田, 恭子× 佐藤, 健司× 中村, 考志 |
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抄録(日) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 抹茶中の総アミノ酸および遊離アミノ酸をリチウムバッファー系のHPLC-アミノ酸分析機で定量した。総アミノ酸は抹茶を1.0Φ×5cm試験管に入れ, ミニナートバルプ容器(25ml)中で6NHCl蒸気の気相加水分解法によった。遊離アミノ酸は試料抹茶中に2%含まれ, そのうちテアニンが最も多く含まれていた。結合型アミノ酸(蛋白態またはペプチド態アミノ酸)は約14.7%であった。本アミノ酸分析機の検出器は, UV-VIS型であるため, ニンヒドリン反応の停止した状態で, 検出波長を254nm, 280nm, 556nm等で検出でき, 未知着色物質や紫外吸収物質の検出, 分取に利用できる。 | |||||
言語 | ja | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Free amino acids and total amino acids (6N-HCL hydrolysate at 150℃ for 1hr) in Mat-cha (powdered green tea) were determined with an HPLC-amino acid analyzer using lithium citrate buffers. The Mat-cha contained 2% of free amino acid and about 14.7% of bound amino acid (proteins and peptides). Theanine was the most abundant free amino acid. When the ninhydrin-reagent pump was stopped, the effluent from the HPLC-a.a.analyzer was monitored with a UV-VIS detector at 254nm, 280nm, and 556nm to detect and to collect the unknow compounds of the Mat-cha extract. | |||||
言語 | en | |||||
書誌情報 |
ja : 京都府立大学学術報告. 人間環境学・農学 en : The scientific reports of Kyoto Prefectural University. Human environment and agriculture 巻 50, p. 27-31, 発行日 1998-12-25 |
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雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11189254 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 13433954 |