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葯培養によるトウモロコシの半数体植物の育成 I : カルスの形成と根の分化について(農学部門)
https://kpu.repo.nii.ac.jp/records/4898
https://kpu.repo.nii.ac.jp/records/4898aeecb1c9-438c-42c3-96e2-10699f6c48c1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000078897.pdf (1.6 MB)
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| Item type | [ELS]紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-02-20 | |||||
| タイトル | ||||||
| タイトル | 葯培養によるトウモロコシの半数体植物の育成 I : カルスの形成と根の分化について(農学部門) | |||||
| 言語 | ja | |||||
| その他（別言語等） | ||||||
| その他のタイトル | Induction of haploid plant by anther culture in maize I : On the callus formation and root differentiation (Agriculture) | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
村上, 道夫
× 村上, 道夫× 高橋, 信夫× 原田, 賢之 |
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| 抄録(日) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | トウモロコシの葯培養による半数体植物育成の可能性を検討するために, 1核期の花粉をふくむ葯を用いて, 変更Millerおよび変更Whiteの基本培地にauxinsならびにcytokininsを種々の割合に添加した培地に培養し, 培養初期における葯の形態的変化, カルス形成さらに器官分化の様相について追跡調査した。培養初期における葯形態の変化中, 変色枯死する葯はMiller培地で多く20∿40%である。逆に開裂葯はWhite培地で多く50∿64%に達し, 葯の肥大化はMiller培地上で10∿20%に達し, これらよりはいずれもカルス形成は認められない。培養後20日を経過した無変化の葯より, 後にカルス形成が認められる。カルス形成率は0.04%できわめて低い。カルスの形態は均一でなく, 肥大速度にも差異が認められるが概して緩慢である。カルスの移植培養によって根の分化が認められたが, 発根程度ならびにその速度は培養条件によって著しく影響されるが, 一般に伸長速度はおそい。半数性カルスならびに根の存在を確認することが出来ず, また茎葉分化が認められなかったが, 本実験の結果, トウモロコシのカルス形成ならびに根の分化は可能であり, 半数体植物育成の可能性を類推させた。 | |||||
| 言語 | ja | |||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | This experiment was made a trial for investigate possibility on induction of haploid plant by anther culture in maize (Zea mays L.). Several anthers with uni-nucleate stage of pollen from male flowers, 7∿10 days before heading, were placed on the medium. The basic media were prepared modified Whites medium and modified Miller's medium containing various concentration of 2,4-D, IAA and kinetin. The media were adjusted to pH5.8. Among the morphological variation of anthers placed on the media, anthers changed brown and died after a while were amounted about 20∿40% of all placed ones on Miller's media, and dehiscent anthers and enlarged ones were observed 50∿64% on White's media and 10∿20% on Miller's media respectively. Primordium of callus was formed in the ends of anthers inchanged in about 20 days after anther culture, and decided callus formation was recognized in extremely few anthers in 4∿8 weeks after anther placing. The percentage of callus formation was about 0.04%. The callus formed by anther culture had not uniformity in the size and colour, and generally the state of callus increased was very inactivity. Callus formed on several media were transplanted on modified Miller's media in supplement with various auxins and kinetin. Degree of rooting from callus and it's velocity of growth were susceptible to influences from the conditions of media. In general, state of rooting was very inactivity on all media and the optimum constituents of media for root differentiation were yet to be determined through result of above experiments. Roots with haploid number of chromosomes were well not ascertained and the differentiation of leaves and stem from callus were not recognized to the last of these anther culture. From these investigation mentioned above, it was recognized that it is difficult to make the haploid plant of maize by anther culture. Our experimental results show, however, that the callus formation and root differentiation from callus were successful. We expect to establish, therefore, available method for induction of haploid maize by improving the method of anther culture. | |||||
| 言語 | en | |||||
| 書誌情報 |
ja : 京都府立大學學術報告. 農學 en : The scientific reports of Kyoto Prefectural University. Agriculture 巻 24, p. 1-"8-3", 発行日 1972-10-15 |
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| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00062275 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 00757373 | |||||
