{"created":"2023-06-19T08:46:37.317999+00:00","id":5525,"links":{},"metadata":{"_buckets":{"deposit":"d5b4667b-2986-4209-9414-e01badf55e87"},"_deposit":{"created_by":14,"id":"5525","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"5525"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00005525","sets":["47:274:464"]},"author_link":["6353","9755","10258","9757"],"control_number":"5525","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"Separation and Purification of Kiwifruit Protease : Purification with Bakerbond WP-PEI (B. LIVING SCIENCE)","subitem_alternative_title_language":"en"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-11-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"41","bibliographicPageStart":"37","bibliographicVolumeNumber":"44","bibliographic_titles":[{"bibliographic_title":"京都府立大学学術報告. 理学・生活科学","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of the Kyoto Prefectural University. Natural science and living science","bibliographic_titleLang":"en"}]}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Kiwifruit protease (EC 3. 4. 22. 14) was purified with conventional DEAE-cellulose and Octyl-Sepharose, a packing material of hydrophobic chromatography. With these methods, a rapid purification or concentration of the enzyme was obtained. The partially purified protease was further purified and successfully separated on the column of Bakerbond WP-PEI with a lineargradient elution of 0-0.75M NaCl into two enzymes, A_1 and A_2,which were named by Mcdowall (Biochim. Biophys. Acta, 293,226 (1973)). Kiwifruit proteases A_1 and A_2 were purified to 90% and 93% homogeneity of mol. wt. as checked by TSK G2000SW XL gel-chromatography.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062300","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0075739X","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"大槻, 耕三","creatorNameLang":"ja"},{"creatorName":"オオツキ, コウゾウ","creatorNameLang":"ja-Kana"},{"creatorName":"Otsuki, Kozo","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"三宅, 智恵子","creatorNameLang":"ja"},{"creatorName":"ミヤケ, チエコ","creatorNameLang":"ja-Kana"},{"creatorName":"Miyake, Chieko","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"佐藤, 健司","creatorNameLang":"ja"},{"creatorName":"サトウ, ケンジ","creatorNameLang":"ja-Kana"},{"creatorName":"Sato, Kenji","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"河端, 信","creatorNameLang":"ja"},{"creatorName":"カワバタ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"Kawabata, Makoto","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000079651.pdf","filesize":[{"value":"300.0 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000079651.pdf","url":"https://kpu.repo.nii.ac.jp/record/5525/files/KJ00000079651.pdf"},"version_id":"5bd37adb-be11-4947-995e-fa7f4260e9ee"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"キーウィフルーツ・プロテアーゼの分離・精製 : 新しいイオン交換充填剤 Bakerbond WP-PEI による分離(B. 生活科学)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"キーウィフルーツ・プロテアーゼの分離・精製 : 新しいイオン交換充填剤 Bakerbond WP-PEI による分離(B. 生活科学)","subitem_title_language":"ja"}]},"item_type_id":"3","owner":"14","path":["464"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"5525","relation_version_is_last":true,"title":["キーウィフルーツ・プロテアーゼの分離・精製 : 新しいイオン交換充填剤 Bakerbond WP-PEI による分離(B. 生活科学)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-06-06T04:27:55.724128+00:00"}