{"created":"2023-06-19T08:46:34.656654+00:00","id":5464,"links":{},"metadata":{"_buckets":{"deposit":"30222e78-5451-456c-aa1b-e43acbc0e55c"},"_deposit":{"created_by":14,"id":"5464","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"5464"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00005464","sets":["47:274:460"]},"author_link":["9959"],"control_number":"5464","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"The Measurement of Association Equilibrium Constants of Trypsins with a Protein Proteinase Inhibitor (B. LIVING SCIENCE)","subitem_alternative_title_language":"en"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1989-11-18","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"18","bibliographicPageStart":"13","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"京都府立大学学術報告. 理学・生活科学","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of the Kyoto Prefectural University. Natural science and living science","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"トリプシンとインヒビターの会合平衡定数測定のための改良法を確立した。本法は3段階より成っており, 1段階は, 活性部位滴定や酵素-インヒビター滴定によるトリプシンおよびインヒビターの濃度標定, 2段階は, GreenとWork法での滴定のための遊離酵素活性の正確な測定で, ここでは, 低酵素濃度と高感度基質が用いられる。最後の段階は, マイクロ・コンピューターによるデータの非線型最小自乗分析で, これにより正確に定数を評価できうる。この方法により, ウシおよびブタ・トリプシンと粟種子トリプシンインヒビターIIとの会合平衡定数は, 25℃, pH8.0で, それぞれ3.3×10^<10>M^<-1>, 4.6×10^9M^<-1>と決定された。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"An improved procedure for the measurement of association equilibrium constants of trypsins with a protein proteinase inhibitor has been established. The procedure consists of three stages : (a) standardization of trypsin and inhibtor solutions by the active-site titration with p-nitrophenyl-p'-guanidinobenzoate and by an enzyme-inhibitor titration at a high concentration (&acd;10^<-7>M), (b) second is the accurate measurement of free enzyme activities for Green and Work titrations, where low enzyme concentrations (&acd;10^<-10>M) and a highly sensitive substrate are used, and (c) nonlinear least-squares analyses of the data by a microcomputer, which make it possible to accurately estimate the constants. By this procedure, the association equilibrium constants of bovine and hog trypsins with foxtail millet trypsin inhibitor II were determined to be 3.3×10^<10>M^<-1> and 4.6×10^9M^<-1>, respectively, at 25℃ and pH 8.0.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062300","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0075739X","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田代, 操","creatorNameLang":"ja"},{"creatorName":"タシロ, ミサオ","creatorNameLang":"ja-Kana"},{"creatorName":"Tashiro, Misao","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000079590.pdf","filesize":[{"value":"435.4 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000079590.pdf","url":"https://kpu.repo.nii.ac.jp/record/5464/files/KJ00000079590.pdf"},"version_id":"cbc1dee5-d500-4989-a987-9f3d84cca8d9"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"蛋白質性プロテアーゼインヒビターとトリプシンの会合平衡定数測定(B. 生活科学)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"蛋白質性プロテアーゼインヒビターとトリプシンの会合平衡定数測定(B. 生活科学)","subitem_title_language":"ja"}]},"item_type_id":"3","owner":"14","path":["460"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"5464","relation_version_is_last":true,"title":["蛋白質性プロテアーゼインヒビターとトリプシンの会合平衡定数測定(B. 生活科学)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-01-19T05:00:51.818772+00:00"}