{"created":"2023-06-19T08:46:34.104878+00:00","id":5451,"links":{},"metadata":{"_buckets":{"deposit":"121510ce-e191-4360-a22a-7a761bdf868e"},"_deposit":{"created_by":4,"id":"5451","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"5451"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00005451","sets":["47:274:459"]},"author_link":["9960","9959"],"control_number":"5451","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"The Cleavage of Asp (19)-Pro (20) Bond in Rice Bran Trypsin Inhibitor under Acidic Condition (B. LIVING SCIENCE)","subitem_alternative_title_language":"en"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1988-11-18","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"34","bibliographicPageStart":"29","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"京都府立大学学術報告. 理学・生活科学","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of the Kyoto Prefectural University. Natural science and living science","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"米糠トリプシンインヒビター(RBTI)分子中のAsp (19)-Pro (20)結合の開裂が酸性pH下で非酵素的に生じた。このことは,pH3.0でのRBTIのインキュベーション,開裂したペプチド結合を有する修飾RBTIの単離,さらに修飾インヒビターの還元およびS-カルボキシメチル化によって得られたペプチドの分析などにより明らかにされた。修飾RBTIのトリプシン阻害活性は未修飾のものに比べ著しく低下しており,これはRBTI分子中のAsp (19)-Pro (20)結合の開裂により阻害活性部位周辺の立体構造に変動が生じたためであると考えられた。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"The cleavage of Asp (19)-Pro (20) bond in rice bran trypsin inhibitor (RBTI) occurred non-enzymatically at an acid pH. This was proved by the experiments such as incubation of RBTI at pH 3.0,isolation of a modified RBTI containing a cleaved peptide bond, and chemical analyses of peptides obtained by reduction and S-carboxymethylation of the modified inhibitor. The trypsin inhibitory activity of the modified RBTI was dramatically weaker than that of native RBTI, suggesting that the cleavage of the Asp (19)-Pro (20) bond in RBTI caused a conformational change in the structure of a reactive-site region.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062300","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0075739X","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田代, 操","creatorNameLang":"ja"},{"creatorName":"タシロ, ミサオ","creatorNameLang":"ja-Kana"},{"creatorName":"Tashiro, Misao","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"牧, 善輔","creatorNameLang":"ja"},{"creatorName":"マキ, ゼンスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Maki, Zensuke","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000079577.pdf","filesize":[{"value":"621.2 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000079577.pdf","url":"https://kpu.repo.nii.ac.jp/record/5451/files/KJ00000079577.pdf"},"version_id":"14879f67-e72f-4b95-a4eb-a852dd4bbd83"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"酸性条件下における米糠トリプシンインヒビターの Asp (19)-Pro (20) 結合の開裂(B. 生活科学)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"酸性条件下における米糠トリプシンインヒビターの Asp (19)-Pro (20) 結合の開裂(B. 生活科学)","subitem_title_language":"ja"}]},"item_type_id":"3","owner":"4","path":["459"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"5451","relation_version_is_last":true,"title":["酸性条件下における米糠トリプシンインヒビターの Asp (19)-Pro (20) 結合の開裂(B. 生活科学)"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2024-02-29T05:14:33.088375+00:00"}