{"created":"2023-06-19T08:46:29.998096+00:00","id":5359,"links":{},"metadata":{"_buckets":{"deposit":"1e6a663b-612a-4d8c-82b8-8e2c0741e3dd"},"_deposit":{"created_by":14,"id":"5359","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"5359"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00005359","sets":["47:274:452"]},"author_link":["9745"],"control_number":"5359","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"ササゲ (Vigna sinensis) の組織培養におけるカルス形成, 継代培養および馴化について(A. 理学)","subitem_alternative_title_language":"ja"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1981-11-20","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"9","bibliographicPageStart":"1","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"京都府立大学学術報告. 理学・生活科学","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of the Kyoto Prefectural University. Natural science and living science","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"ササゲ(Vigna sinensis Endl.)組織培養のカルス形成, 継代培養, 馴化について調べた。子葉, 胚軸, 根の切片をIAAか2,4-Dを含むLinsmaier-Skoog培地で培養するとカルスを形成した。カルス形成はIAAより2,4-Dの方が強い活性を示した。子葉の切片は胚軸や根の切片より高濃度のオーキシンを必要とした。カイネチンおよびニコチン酸はカルス形成に大きな効果を示さなかった。酵母抽出物はオーキシンおよびカイネチンと共に使用すると, 旺盛なカルス形成をひこおこした。継代培養では, 2,4-Dを含む培地では黄色の柔らかいカルス組織を生じ, 発根は見られなかった。一方。オーキシンとしてIAAを含む培地では組織は発根し褐色を呈した。多数の分化した細胞を含むためと考えられる。カイネチンは組織の生長を促進し, さらに組織の性状に影響した。低濃度のカイネチンでは柔らかい組織を生じたが, 高濃度のカイネチンは硬い組織を生じた。逆に高濃度のオーキシンは柔らかい組織を生じた。オーキシンおよびサイトカイニンの量はカルス組織の細胞の分化の程度を支配していることを示すものと考えられる。ニコチン酸および酵母抽出物はカルス組織の生成を著しく促進するが, 組織の性状に影響しなかった。2,4-Dおよびカイネチン, ニコチン酸, 酵母抽出物を選択的に加えた培地で15回以上継代培養したカルス組織から, これらの生長因子に対する要求性の異なったカルス組織が少なくとも3種得られた。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Tissue culture of Vigna sinensis Endl. was studied with reference to callus formation, subculture and habituation. Callus formation was observed on cotyledon, hypocotyl and root sections, when cultured on Linsmaier and Skoog basal medium supplemented with IAA or 2,4-D. 2,4-D showed higher stimulative activity than IAA. Cotyledon sections required higher auxin level for callus formation than hypocotyl and root ones. Kinetin and nicotinic acid were not so effective to the callus formation. Yeast extract caused vigorous callus formation, when used with auxin and kinetin. No bud formation was observed in any section on any medium. In the subculture of the callus, IAA (10^<-7>&acd;4×10^<-5>M) gave brown callus with roots, possibly containing differentiated tracheal elements, while 2,4-D (except 10^<-8> and 10^<-9>M) did yellow friable and homogenous callus without root. Kinetin somewhat promoted the callus growth, and significantly affected the tissue texture. High level of kinetin gave compact callus, while high level of auxin did friable callus. Nicotinic acid promoted much the callus growth, but had no effect on the tissue texture. Yeast extract strongly stimulated the callus growth. After more than 15 transfers of subculture in the media added with 2,4-D, kinetin, nicotinic acid and yeast extract, alone or in combination, at least three distinguishable stock calli with different requirement for growth factors were obtained.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062300","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0075739X","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"松原, 聰","creatorNameLang":"ja"},{"creatorName":"マツバラ, サトシ","creatorNameLang":"ja-Kana"},{"creatorName":"Matsubara, Satoshi","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000079485.pdf","filesize":[{"value":"726.7 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000079485.pdf","url":"https://kpu.repo.nii.ac.jp/record/5359/files/KJ00000079485.pdf"},"version_id":"d4c3629f-065e-4e8a-818b-678e061e1ccc"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Studies on Tissue Culture of Vigna sinensis Endl. with Reference to Callus Formation, Subculture and Habituation (A. NATURAL SCIENCE)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Studies on Tissue Culture of Vigna sinensis Endl. with Reference to Callus Formation, Subculture and Habituation (A. NATURAL SCIENCE)","subitem_title_language":"en"}]},"item_type_id":"3","owner":"14","path":["452"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"5359","relation_version_is_last":true,"title":["Studies on Tissue Culture of Vigna sinensis Endl. with Reference to Callus Formation, Subculture and Habituation (A. NATURAL SCIENCE)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-02-29T04:51:27.413138+00:00"}