{"created":"2023-06-19T08:46:28.524136+00:00","id":5325,"links":{},"metadata":{"_buckets":{"deposit":"57891a81-74bd-4287-bb77-626bb0e138d0"},"_deposit":{"created_by":14,"id":"5325","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"5325"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00005325","sets":["47:274:449"]},"author_link":["9756","9755","9757"],"control_number":"5325","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"Purification and Stabilization of Ginger Protease (B. LIVING SCIENCE)","subitem_alternative_title_language":"en"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1978-11-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"40","bibliographicPageStart":"33","bibliographicVolumeNumber":"29","bibliographic_titles":[{"bibliographic_title":"京都府立大学学術報告. 理学・生活科学","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of the Kyoto Prefectural University. Natural science and living science","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"ショウガの根に含まれる蛋白質加水分解酵素をアセトン処理, りん酸緩衝液抽出, CM-セルロースクロマトグラフィー, Bio-Gel P-100によるゲルクロマトグラフィーなどの処理により高純度に精製した.この際プロテアーゼの自己消化防止剤(安定化剤)として, HgCl_2とPCMBの水銀剤を使用しその効果を比較検討したが, HgCl_2を反応させScheme 1の方法で精製した酵素は, 二量体の酵素が生成していて, Bio-Gel P-100のクロマトグラム上で高分子の不純物と重り合った.PCMBで安定化した酵素をCM-セルロースクロマトグラフィーおよびBio-Gel P-100のゲルクロマトグラフィーにかけて得た標品は最も比活性も大きく純度の高いものであった.この標品は安定化剤を使用しないで精製し得られた標品の約6倍活性の強いものであった.またこの精製プロテアーゼのBio-Gel P-100による分子量の測定によると, 2万9千であって, この値はSDS-スラブ-ポリアクリルアミドゲル電気泳動によって求めた前回の実験値とよく一致した.","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Ginger protease which exists in the rhyzome (Zingiber officinale roscoe) was purified by chromatography on CM-cellulose and gel filtration on Bio-Gel P-100. In the course of the purification, HgCl_2 or p-chloromercuribenzoic acid (PCMB) was used as a stabilizing agent for the protease. It was shown that the protease stabilized with HgCl_2 formed a dimer complex which could not be separated from the higher molecular contaminant. The protease activity of the preparation stabilized with PCMB and purified by the chromatography was as high as 6-fold of that without PCMB. From the result of gel filtration on Bio-Gel P-100,the molecular weight of this purified protease was estimated to be about 29,000,which is the same as the value obtained previously by SDS slab-polyacrylamide gel electrophoresis.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062300","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0075739X","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"大槻, 耕三","creatorNameLang":"ja"},{"creatorName":"オオツキ, コウゾウ","creatorNameLang":"ja-Kana"},{"creatorName":"Otsuki, Kozo","creatorNameLang":"en"}],"familyNames":[{"familyName":"大槻","familyNameLang":"ja"},{"familyName":"オオツキ","familyNameLang":"ja-Kana"},{"familyName":"Otsuki","familyNameLang":"en"}],"givenNames":[{"givenName":"耕三","givenNameLang":"ja"},{"givenName":"コウゾウ","givenNameLang":"ja-Kana"},{"givenName":"Kozo","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"9757","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"河端, 信","creatorNameLang":"ja"},{"creatorName":"カワバタ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"Kawabata, Makoto","creatorNameLang":"en"}],"familyNames":[{"familyName":"河端","familyNameLang":"ja"},{"familyName":"カワバタ","familyNameLang":"ja-Kana"},{"familyName":"Kawabata","familyNameLang":"en"}],"givenNames":[{"givenName":"信","givenNameLang":"ja"},{"givenName":"マコト","givenNameLang":"ja-Kana"},{"givenName":"Makoto","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"9755","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"田口, 邦子","creatorNameLang":"ja"},{"creatorName":"タグチ, クニコ","creatorNameLang":"ja-Kana"},{"creatorName":"Taguchi, Kuniko","creatorNameLang":"en"}],"familyNames":[{"familyName":"田口","familyNameLang":"ja"},{"familyName":"タグチ","familyNameLang":"ja-Kana"},{"familyName":"Taguchi","familyNameLang":"en"}],"givenNames":[{"givenName":"邦子","givenNameLang":"ja"},{"givenName":"クニコ","givenNameLang":"ja-Kana"},{"givenName":"Kuniko","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"9756","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000079451.pdf","filesize":[{"value":"715.6 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000079451.pdf","url":"https://kpu.repo.nii.ac.jp/record/5325/files/KJ00000079451.pdf"},"version_id":"457abee7-dff9-4102-8ed7-164c3ffd9f76"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ショウガ・プロテアーゼの精製と安定化(B. 生活科学)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ショウガ・プロテアーゼの精製と安定化(B. 生活科学)","subitem_title_language":"ja"}]},"item_type_id":"3","owner":"14","path":["449"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"5325","relation_version_is_last":true,"title":["ショウガ・プロテアーゼの精製と安定化(B. 生活科学)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-06-06T05:09:44.233070+00:00"}