{"created":"2023-06-19T08:46:23.316102+00:00","id":5206,"links":{},"metadata":{"_buckets":{"deposit":"ff9d3f9b-9aee-483f-9fe5-465b17db1fdb"},"_deposit":{"created_by":14,"id":"5206","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"5206"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00005206","sets":["47:273:437"]},"author_link":["9191","9362","9125"],"control_number":"5206","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"Construction of the rice genomic P1 library (Agricultural Chemistry)","subitem_alternative_title_language":"en"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-12-08","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"33","bibliographicPageStart":"26","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"京都府立大學學術報告. 農學","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of Kyoto Prefectural University. Agriculture","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"プロトプラスト化せず, イネ若葉より1Mb程度の高分子量核DNAを調製する手法を確立した。制限酵素Mbo Iにより部分消化した後, 70-95kbのイネゲノムDNA断片を得た。このDNAを材料として, P1クローニングシステムを用いてイネゲノムライブラリーを構築した。任意に選択したクローンよりプラスミドを回収し, 制限酵素Not Iで切断し電気泳動した結果, このライブラリーは平均85kbのインサートDNAを持つプラスミドによって構成されていることが分かった。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"We constructed a P1 library containing 70-95kb inserts of rice genomic DNA. The high molecular weight DNA was obtained from rice young leaves by a simple liquid nitrogen method. The high molecular weight DNAs were partially digested with restriction enzyme Mbo I, and ligated into P1 vector. This library consisted about 2,000 individual clones, and was expected to be useful for large scale genomic analyses.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062275","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00757373","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田中, 國介","creatorNameLang":"ja"},{"creatorName":"タナカ, クニスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanaka, Kunisuke","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"牧野, 英志","creatorNameLang":"ja"},{"creatorName":"マキノ, ヒデシ","creatorNameLang":"ja-Kana"},{"creatorName":"Makino, Hideshi","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"増村, 威宏","creatorNameLang":"ja"},{"creatorName":"マスムラ, タケヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Masumura, Takehiro","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000079205.pdf","filesize":[{"value":"644.9 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000079205.pdf","url":"https://kpu.repo.nii.ac.jp/record/5206/files/KJ00000079205.pdf"},"version_id":"a6606e9e-69b2-4120-961c-622e3955e1bb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"イネ(日本晴)ゲノム P1 ライブラリーの構築(農芸化学部門)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"イネ(日本晴)ゲノム P1 ライブラリーの構築(農芸化学部門)","subitem_title_language":"ja"}]},"item_type_id":"3","owner":"14","path":["437"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"5206","relation_version_is_last":true,"title":["イネ(日本晴)ゲノム P1 ライブラリーの構築(農芸化学部門)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-03-01T00:59:24.496407+00:00"}