{"created":"2023-06-19T08:46:21.980138+00:00","id":5175,"links":{},"metadata":{"_buckets":{"deposit":"d446b888-303a-4ad0-b3d6-08755c8b4d0d"},"_deposit":{"created_by":14,"id":"5175","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"5175"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00005175","sets":["47:273:433"]},"author_link":["9125","9191","9286","7963"],"control_number":"5175","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"Construction of cDNA library using electro-transformation(Agricultural Chemistry)","subitem_alternative_title_language":"en"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1989-11-18","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"92","bibliographicPageStart":"86","bibliographicVolumeNumber":"41","bibliographic_titles":[{"bibliographic_title":"京都府立大學學術報告. 農學","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of Kyoto Prefectural University. Agriculture","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"電気的形質転換法を用いプラスミドDNAを導入することにより大腸菌を形質転換した。我々は高効率を得る工夫をし, この方法を用いることでコンピテントセルを用いる形質転換法(Hanahan法)では出せない高効率を得ることが可能になった。ここではこの方法で登熟期イネ胚乳及びホウレンソウ芽生えよりcDNAライブラリーの作製に成功した。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"To transform Escherichia coli a plasmid DNA was introduced by electroporation. We improved transformation efficiency adding several chemicals, biological materials and/or preheating of the buffer solution which was added just affer electrodischarge. The improved method gave higher transformation efficiency comparing with that of Hanahan's. cDNA libraries for mRNAs appearing in the developing rice seeds and spinach seedlings were constructed using this method.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062275","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00757373","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田中, 國介","creatorNameLang":"ja"},{"creatorName":"タナカ, クニスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanaka, Kunisuke","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"若浦, 誠","creatorNameLang":"ja"},{"creatorName":"ワカウラ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"Wakaura, Makoto","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"増村, 威宏","creatorNameLang":"ja"},{"creatorName":"マスムラ, タケヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Masumura, Takehiro","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"藤井, 昭治","creatorNameLang":"ja"},{"creatorName":"フジイ, ショウジ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujii, Shoji","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000079174.pdf","filesize":[{"value":"400.6 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000079174.pdf","url":"https://kpu.repo.nii.ac.jp/record/5175/files/KJ00000079174.pdf"},"version_id":"afef28e0-8cf7-4383-a50b-3fefd2c5aab1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"電気的形質転換法を用いた cDNA ライブラリーの作製(農芸化学部門)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"電気的形質転換法を用いた cDNA ライブラリーの作製(農芸化学部門)","subitem_title_language":"ja"}]},"item_type_id":"3","owner":"14","path":["433"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"5175","relation_version_is_last":true,"title":["電気的形質転換法を用いた cDNA ライブラリーの作製(農芸化学部門)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-03-21T04:25:38.401653+00:00"}