{"created":"2023-06-19T08:46:20.949967+00:00","id":5151,"links":{},"metadata":{"_buckets":{"deposit":"e4f64f70-b43e-4ac0-950c-70f3385b40e3"},"_deposit":{"created_by":14,"id":"5151","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"5151"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00005151","sets":["47:273:431"]},"author_link":["9189","9125","9749","7963","9191","9188","9187","9186"],"control_number":"5151","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"Immunological screening of cDNA clones using electroblotting (Agricultural Chemistry)","subitem_alternative_title_language":"en"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1987-11-18","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"111","bibliographicPageStart":"102","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"京都府立大學學術報告. 農學","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of Kyoto Prefectural University. Agriculture","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"大腸菌発現ベクターpUC 9を用いて作製したcDNAライブラリーから電気的ブロッティング法を用いる抗体スクリーニングを行った。ここに示す電気的ブロッティング法を使用すると, 従来の抗体スクリーニング法で障害となる非特異的吸着によるバックグラウンドをほとんど除去出来た。ここでは, 登熟期イネ種子について作製したcDNAライブラリーより, プロラミン抗体を用いてプロラミンcDNAの一つをスクリーニングする過程を例として示し, この方法の特徴を解説する。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"A cDNA library constructed using plasmid expression vector, pUC 9,was screened immunologically after the products of the transformed cells were electrically transfered to nitrocellulose filter. Electroblotting technic mentioned here reduced remarkably the background which interfered strongly the identification of the target clones. In this paper, an example is shown in which a clone carring rice prolamin cDNA sequence was screened from a cDNA library for developing rice seed using the electroblotting technic.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062275","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00757373","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"増村, 威宏","creatorNameLang":"ja"},{"creatorName":"マスムラ, タケヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Masumura, Takehiro","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"北村, 敦則","creatorNameLang":"ja"},{"creatorName":"キタムラ, アツノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Kitamura, Atsunori","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"日尾野, 隆","creatorNameLang":"ja"},{"creatorName":"ヒビノ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"Hibino, Takashi","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"多田, 三樹","creatorNameLang":"ja"},{"creatorName":"タダ, ミキ","creatorNameLang":"ja-Kana"},{"creatorName":"Tada, Miki","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"柴田, 大輔","creatorNameLang":"ja"},{"creatorName":"シバタ, ダイスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Shibata, Daisuke","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"竹葉, 剛","creatorNameLang":"ja"},{"creatorName":"タケバ, ゴウ","creatorNameLang":"ja-Kana"},{"creatorName":"Takeba, Go","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田中, 國介","creatorNameLang":"ja"},{"creatorName":"タナカ, クニスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanaka, Kunisuke","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"藤井, 昭治","creatorNameLang":"ja"},{"creatorName":"フジイ, ショウジ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujii, Shoji","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000079150.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000079150.pdf","url":"https://kpu.repo.nii.ac.jp/record/5151/files/KJ00000079150.pdf"},"version_id":"5918ce73-c976-4c20-9b9a-61e918d7ecdb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"電気的ブロッティング法を用いる cDNA クローンの抗体スクリーニング(農芸化学部門)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"電気的ブロッティング法を用いる cDNA クローンの抗体スクリーニング(農芸化学部門)","subitem_title_language":"ja"}]},"item_type_id":"3","owner":"14","path":["431"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"5151","relation_version_is_last":true,"title":["電気的ブロッティング法を用いる cDNA クローンの抗体スクリーニング(農芸化学部門)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-03-21T04:25:38.608738+00:00"}