{"created":"2023-06-19T08:46:20.906758+00:00","id":5150,"links":{},"metadata":{"_buckets":{"deposit":"b70e1a74-a05d-4717-ad70-aac680fd1a67"},"_deposit":{"created_by":14,"id":"5150","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"5150"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00005150","sets":["47:273:431"]},"author_link":["9191","9182"],"control_number":"5150","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"ラムダベクターを用いたイネ遺伝子ライブラリーの作製(農芸化学部門)","subitem_alternative_title_language":"ja"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1987-11-18","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"101","bibliographicPageStart":"95","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"京都府立大學學術報告. 農學","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of Kyoto Prefectural University. Agriculture","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"バクテリオファージラムダベクターEMBL 3を用いた遺伝子ライブラリーの作製法を植物のDNAに対して効率が上がるように改変した。核DANはイネ胚芽より調製したが, 制限酵素の基質とするためには, セシウムクロライド平衡密度勾配遠心で精製する必要があった。単離したDNAはMbo Iで部分分解し, 主にFrischaufら(J. Mol. Biol. 170 827 (1983))の方法により, バクテリオファージラムダベクターEMBL 3アームと結合した。バクテリオファージDNAとイネ胚芽DNAの結合効率は, ポリエチレングリコール(PEG)6000の添加で上昇した。更に, PEG6000はin vitroパッケージングの効率も上げた。結合反応とパッケージングに対するこの改良された方法は, 植物の遺伝子ライブラリー作製に一般的に適用することが可能であろう。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"The method for construction of genomic library using a bacteriophage lambda vector EMBL 3 was modified so as to increase its efficiency for plant DNA. Genomic DNA was prepared from rice embryos, but to be a substrate of a restriction enzyme it had to be purified by equilibrium centrifugation in a cesium chloride density gradient. The isolated DNA was partially digested with Mbo I, then ligated with bacteriophage lambda vector EMBL 3 arms substantially according to Frischauf et al. (J. Mol. Biol. 170,827 (1983)). The efficiency for ligation between the bacteriophage DNA and the rice embryo DNA was improved by addition of polyethylen glycol (PEG) 6000. Moreover the use of PEG 6000 raised the efficiency of in vitro packaging. This improved procedure for the ligation and packaging of the recombinant DNA will be generally applicable to the construction of plant genomic libraries.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062275","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00757373","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田中, 國介","creatorNameLang":"ja"},{"creatorName":"タナカ, クニスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanaka, Kunisuke","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松井, 健二","creatorNameLang":"ja"},{"creatorName":"マツイ, ケンジ","creatorNameLang":"ja-Kana"},{"creatorName":"Matsui, Kenji","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000079149.pdf","filesize":[{"value":"502.0 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000079149.pdf","url":"https://kpu.repo.nii.ac.jp/record/5150/files/KJ00000079149.pdf"},"version_id":"cf2c2b62-41e7-4a44-89dd-6373da36136e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"bacteriophage lambda vector (EMBL 3)","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Construction of genomic library of rice using a bacteriophage lambda vector (Agricultural Chemistry)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Construction of genomic library of rice using a bacteriophage lambda vector (Agricultural Chemistry)","subitem_title_language":"en"}]},"item_type_id":"3","owner":"14","path":["431"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"5150","relation_version_is_last":true,"title":["Construction of genomic library of rice using a bacteriophage lambda vector (Agricultural Chemistry)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-03-05T08:11:32.716062+00:00"}