{"created":"2023-06-19T08:46:20.306288+00:00","id":5136,"links":{},"metadata":{"_buckets":{"deposit":"3e27f835-080d-4fd3-9220-9de021d7ebca"},"_deposit":{"created_by":14,"id":"5136","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"5136"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00005136","sets":["47:273:430"]},"author_link":["9125","9749","9128","7963","9191","9127","9126"],"control_number":"5136","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"A simplified microinjection method for protein biosynthesis in Xenopus Oocytes (Agricultural Chemistry)","subitem_alternative_title_language":"en"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1986-11-15","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"26","bibliographicPageStart":"17","bibliographicVolumeNumber":"38","bibliographic_titles":[{"bibliographic_title":"京都府立大學學術報告. 農學","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of Kyoto Prefectural University. Agriculture","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"アフリカツメガエル卵母細胞へのRNA, DNA等の細胞成分注入用の簡易な微量注入装置を考案, 試作した。この装置を用い特に植物性mRNAをアフリカツメガエル卵母細胞へ注入しタンパク質生合成を行わせた。その結果, tobacco mosaic virus RNA, brome mosaic virus RNA, イネglutelin前駆体mRNA, エンドウriburose 1,5 bis-phosphate carboxylase mRNAが忠実に翻訳され, 対応するpolypeptideが形成されることをsodium dodecyl sulfate polyacrylamide gel電気泳動(SDS-PAGE)による分子量の測定と免疫学的方法を組合わせることにより確認した。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"A simplified and convenient microinjection system to inject cell components such as DNA and RNA into Xenopus oocytes was devised and constructed. By use of this system, RNAs of plants were injected into Xenopus oocytes, then their protein syntheses were examined. Tobacco mosaic virus RNA, brome mosaic virus RNA, rice glutelin precusor mRNA, and pea Riburose 1,5bisphosphate carboxylase mRNA were faithfully translated. The nature of the synthesized polypeptide was confirmed by fluorography using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoelctrophoresis.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062275","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00757373","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"増村, 威宏","creatorNameLang":"ja"},{"creatorName":"マスムラ, タケヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Masumura, Takehiro","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田嶋, 奈加子","creatorNameLang":"ja"},{"creatorName":"タジマ, ナカコ","creatorNameLang":"ja-Kana"},{"creatorName":"Tajima, Nakako","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"古田, 雅一","creatorNameLang":"ja"},{"creatorName":"フルタ, マサカズ","creatorNameLang":"ja-Kana"},{"creatorName":"Furuta, Masakazu","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山形, 裕士","creatorNameLang":"ja"},{"creatorName":"ヤマガタ, ヒロシ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamagata, Hiroshi","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"竹葉, 剛","creatorNameLang":"ja"},{"creatorName":"タケバ, ゴウ","creatorNameLang":"ja-Kana"},{"creatorName":"Takeba, Go","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田中, 國介","creatorNameLang":"ja"},{"creatorName":"タナカ, クニスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanaka, Kunisuke","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"藤井, 昭治","creatorNameLang":"ja"},{"creatorName":"フジイ, ショウジ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujii, Shoji","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000079135.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000079135.pdf","url":"https://kpu.repo.nii.ac.jp/record/5136/files/KJ00000079135.pdf"},"version_id":"ef2b1eb6-ea14-4dcf-8ad1-fc6dbb38a5ae"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"アフリカツメガエル卵母細胞を用いるタンパク質生合成のための簡易微量注入法(農芸化学部門)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アフリカツメガエル卵母細胞を用いるタンパク質生合成のための簡易微量注入法(農芸化学部門)","subitem_title_language":"ja"}]},"item_type_id":"3","owner":"14","path":["430"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"5136","relation_version_is_last":true,"title":["アフリカツメガエル卵母細胞を用いるタンパク質生合成のための簡易微量注入法(農芸化学部門)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-03-21T04:25:38.822352+00:00"}