@article{oai:kpu.repo.nii.ac.jp:00005118,
 author = {土井, 裕司 and Doi, Hiroshi and 米林, 眞 and Yonebayashi, Makoto and 伊吹, 文男 and Ibuki, Fumio and 金森, 正雄 and Kanamori, Masao},
 journal = {京都府立大學學術報告. 農學, The scientific reports of Kyoto Prefectural University. Agriculture},
 month = {Nov},
 note = {植物性たん白質(主に大豆たん白質)は動物性たん白質(主にカゼイン)に比べて, 血清コレステロール濃度低下作用を有していることが知られている。著者らは先に, 分離大豆たん白質をペプシンで限定水解することによって, その作用を強めることを発見した。大豆たん白質の血清コレステロール濃度低下作用メカニズムを解明するため, コレステロール代謝を考慮すると, 肝臓および小腸の機能を検討することが重要である。本研究は, 分離大豆たん白質食, ペプシン処理分離大豆たん白質食および市販固型食を用いてラットを飼育し, 食餌たん白質のラット肝機能におよぼす影響を比較検討し, 更に, 肝臓および小腸での胆汁酸濃度, 血清遊離アミノ酸パターンを測定した。肝機能を推定するため, 血清中のGlutamate Oxaloacetate Transaminase, Glutamate Pyruvate Transaminase, Lactate Dehydrogenase, Leucine Aminopeptidase, Alkaline Phosphataseの活性を測定した。その結果, 供試食餌たん白質では肝細胞の破壊や胆汁流出障害は観察されなかった。また, 肝内胆汁酸濃度と血清コレステロール濃度とは比例していたが, 小腸胆汁酸濃度には相関がなかった。すなわち, 血清コレステロール濃度調節機構の解明には, 肝機能を更に検討することの必要性が示された。また, トレオニンが, コレステロール代謝と最も関連深いアミノ酸であることが明らかにされた。, It is well-known that vegetable protein (usually soybean protein) has hypocholesterolemic effect compared with animal protein (usually casein). In our previous papers, we indicated that pepsin-treated soybean protein isolate was more hypochole-sterolemic than intact soybean protein isolate. It is important to examine the functions of liver and small intestine in order to make clear the mechanism of hypocholesterolemic effect by dietary soybean protein considering from the point of view of cholesterol metabolism and catabolism. In this paper, effects of dietary protein on liver function and bile acids concentration in liver and small intestine were examined. Rats were fed with soybean protein isolate diet, pepsin-treated soybean protein diet or commercial diet. Serum enzyme acitivities were measured to evaluate the liver function of rats fed various protein diets. Enzymes measured were glutamate-oxaloacetate transaminase, glutamate-pyruvate transaminase, lactate dehydrogenase, leucine aminopeptidase and alkaline phosphatase. In the case of rats fed those diets, the destruction of liver cell and the disease of biliary flow were not observed. Serum cholesterol level was proportion to the bile acids concentration in liver, but not in small intestine. Obtained results suggest the necessity of further examinations of liver function in order to clarify the regulation mechanism of serum cholesterol level by dietary protein. It is indicated from serum aminogram that threonine is closely related to cholesterol mechanism.},
 pages = {26--35},
 title = {ペプシン処理分離大豆たん白質食のラット肝機能および胆汁酸濃度への影響(農芸化学部門)},
 volume = {36},
 year = {1984},
 yomi = {ドイ, ヒロシ and ヨネバヤシ, マコト and イブキ, フミオ and カナモリ, マサオ}
}