@article{oai:kpu.repo.nii.ac.jp:00005080,
 author = {宮田, 善雄 and Miyata, Yoshio and 小田桐, 幸彦 and Odagiri, Yukihiko},
 journal = {京都府立大學學術報告. 農學, The scientific reports of Kyoto Prefectural University. Agriculture},
 month = {Nov},
 note = {クリ疫病菌Phytophthora castaneae KATSURA et UCHIDA CCT 691は, 他の多くの疫病菌と異なり, 極めて高い卵胞子形成能を有し, 卵胞子に関する研究材料として好適である。そこで, 本菌を用いて, 卵胞子を同調的に, 容易に, かつ多量に形成させるための培養方法について検討し, 簡便な手順を完成した。すなわち, 角型培養びんに, 0.1mg/mlのβ-シトステロールを含むキュウリジュース遠沈上清培地をとり, 菌体を振とうあるいは静置培養する。菌体量が最大に達したとき, 培地を棄て, 滅菌脱イオン水で菌体を洗浄し, 培養びんを逆さにして, 菌体が含み得るだけの水分条件として, 24∿26℃定温器内にて, 3∿4日間, さらに培養を行なうと, 菌体乾重1mg当り5×10^4個程度の卵胞子が形成され, その成熟度合も70%に達する。その後は密栓して冷蔵(4℃)すれば, 3カ月以上, 発芽能力は保持され, 任意に実験に供し得る。なお, 培地はステロールを必要量含むものであれば, 既存のいずれのものでもよい。, The pathogenic fungus Phytophthora castaneae CCT691 that causes chestnut trunk rot differs from of the many other Phytophthora in that it produces oospores extremely easily, and is a favorable material for the study of oopores. We developed a culture method of this organism by which a large amount of oospores were easily produced in synchronization. The Phytophthora was cultured by shaking culture in a square vial containing the centrifuged supernatant medium of cucumber juice with 0.1mg/ml of β-sitosterol added. When the fungal body attained its maximum size, the medium was discarded, and the fungal body was washed with sterilized deionized water. The culture vial was then overturned to remove the water except that the fungal body could retain. After a 3- to 4-day dark culture in this water condition, about 5×(10)^4 oospores per 1mg fungal bodies were produced, with a maturation rate of 70%. These oospores retained their ability to germinate for at least 3 months when they were sealed and kept refrigirated (4℃), and could be used for experimentation as needed.},
 pages = {28--34},
 title = {Phytophthora castaneae 卵胞子の多量形成培養法(農学部門)},
 volume = {34},
 year = {1982},
 yomi = {ミヤタ, ヨシオ and オダギリ, ユキヒコ}
}