{"created":"2023-06-19T08:46:12.555533+00:00","id":4966,"links":{},"metadata":{"_buckets":{"deposit":"de4e890a-ca7d-48b3-aabc-cae520df7251"},"_deposit":{"created_by":14,"id":"4966","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"4966"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00004966","sets":["47:273:420"]},"author_link":["7821","7826","8408","8415"],"control_number":"4966","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"数種特異的阻害剤による Phytophthora capsici の無性的分化過程の代謝依存性の推察(農学部門)","subitem_alternative_title_language":"ja"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1976-11-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"62","bibliographicPageStart":"57","bibliographicVolumeNumber":"28","bibliographic_titles":[{"bibliographic_title":"京都府立大學學術報告. 農學","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of Kyoto Prefectural University. Agriculture","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"作用機作がすでに知られている各種特異的阻害剤を用いて, Phytophthora capsiciの無性的生活環における種々の分化の過程の代謝依存性を推察した。RNA合成阻害剤であるアクチノマイシンDは菌糸伸長, 遊走子のう形成, ならびにその発芽を抑制したが, 遊走子の遊泳や被のう胞子の発芽にはほとんど影響しなかった。蛋白合成阻害剤であるシクロヘキシミドはアクチノマイシンDで阻害を受けた過程と, さらに被のう胞子の発芽をも強く阻害した。酸化的リン酸化の阻害剤であるオリゴマイシンはすべての分化過程を阻害したが, 同じ呼吸阻害剤であるアンチマイシンA(電子伝達系阻害剤)は菌糸伸長ならびに被のう胞子の発芽を阻害することができなかった。このことは, それらの過程で電子伝達系が作動していないと考えるよりは, アンチマイシンがそれらの過程において菌の細胞内にとり込まれにくいと考えるほうが妥当である。それと同時に, それらの過程においては物質の透過性をつかさどる菌の原形質膜に何らかの差異があることをも示唆する。このことは菌糸伸長ならびに被のう胞子の発芽過程がナイスタチン(細胞膜のステロール類と結合することにより膜機能を阻害する)に不感受性であることからも示唆される。microtubule阻害剤であるビンブラスチンは遊走子のう形成ならびにその発芽を強く阻害し, また菌糸伸長をも若干阻害した。またmicrofilament阻害剤であるサイトカラシンBは分化のどの過程に対しても顕著な阻害を示さなかった。これらの結果より, それぞれの分化の過程における特定の代謝の依存性が推察された。また, アクチノマイシンDとシクロヘキシミドに対する不感受性によって示された遊走子の遊泳期におけるRNAならびに蛋白合成の不必要は^<14>Cのアミノ酸またヌクレオシドの取り込み実験からも支持された。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Metabolic dependence of differentiation processes during asexual life cycle of Phytophthora capsici was evaluated by the use of specific inhibitors whose modes of the action have been known. Actinomycin D, an RNA synthesis inhibitor, interfered with vegetative growth, and formation and germination of zoosporangia of the fungus, but had negligible adverse effects on swimming of zoospores and germination of encysted zoospores. Cycloheximide, a protein synthesis inhibitor, inhibited germination of encysted zoospores besides the processes inhibited by actinomycin D. Oligomycin, an oxidative phosphorylation inhibitor, inhibited all the differentiation processes. Antimycin A, an electron transport inhibitor, however, failed in inhibiting vegetative growth and germination of encysted zoospores. This was probably due to impermeability of the inhibitor through the plasma membrane and qualitative difference in the membrane at those antimycin A-insensitive phases of the fungus was suggested. The difference in the plasma membrane was also indicated by the sensitivity to nystatin, a membrane sterol inhibitor : nystatin did not inhibit vegetative growth and germination of encysted zoospores, but did more and less other processes. Vinblastin, a microtubule inhibitor, inhibited strongly formation and germination of zoosporangia, and swimming of zoospores, and slightly vegetative growth. None of the differentiation processes evaluated was found to be very sensitive to cytochalasin B, a microfilament inhibitor. From these results, possible requirements of the specific metabolisms or functions for the individual differentiation process was postulated. Furthermore, no requirement of RNA and protein synthesis for swimming of zoospores, as indicated by insensitivity to the inhibitors, was confirmed by tracer experiments.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062275","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00757373","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"吉川, 正明","creatorNameLang":"ja"},{"creatorName":"ヨシカワ, マサアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Yoshikawa, Masaaki","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田中, 裕美","creatorNameLang":"ja"},{"creatorName":"タナカ, ヒロミ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanaka, Hiromi","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"宮田, 善雄","creatorNameLang":"ja"},{"creatorName":"ミヤタ, ヨシオ","creatorNameLang":"ja-Kana"},{"creatorName":"Miyata, Yoshio","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"正子, 朔","creatorNameLang":"ja"},{"creatorName":"マサゴ, ハジメ","creatorNameLang":"ja-Kana"},{"creatorName":"Masago, Hajime","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000078965.pdf","filesize":[{"value":"480.2 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000078965.pdf","url":"https://kpu.repo.nii.ac.jp/record/4966/files/KJ00000078965.pdf"},"version_id":"05a3d0b7-ee3c-4152-9514-c275f05ca36a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Evaluation by specific inhibitors of metabolic dependence of asexual differentiation processes in Phytophthora capsici (Agriculture)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Evaluation by specific inhibitors of metabolic dependence of asexual differentiation processes in Phytophthora capsici (Agriculture)","subitem_title_language":"en"}]},"item_type_id":"3","owner":"14","path":["420"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"4966","relation_version_is_last":true,"title":["Evaluation by specific inhibitors of metabolic dependence of asexual differentiation processes in Phytophthora capsici (Agriculture)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-04-25T04:31:34.643279+00:00"}