{"created":"2023-06-19T08:46:12.513687+00:00","id":4965,"links":{},"metadata":{"_buckets":{"deposit":"b9b8eace-1e96-42dc-b6c9-02bd4b3dc96f"},"_deposit":{"created_by":14,"id":"4965","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"4965"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00004965","sets":["47:273:420"]},"author_link":["7826","8415"],"control_number":"4965","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"ダイズ植物ならびにその病原菌 Phytophthora spp. における poly (A)-containing messenger RNA の存在(農学部門)","subitem_alternative_title_language":"ja"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1976-11-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"56","bibliographicPageStart":"51","bibliographicVolumeNumber":"28","bibliographic_titles":[{"bibliographic_title":"京都府立大學學術報告. 農學","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of Kyoto Prefectural University. Agriculture","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"ダイズ胚軸および2種の植物病原菌, Phytophthora megasperma var. sojaeとP. capsiciを^3H-uridineでパルスラベルし, それらから得たRNA中にpoly (A)-containing messenger RNAが存在するかどうかについて, セファロース4B-poly Uカラムによるaffinityクロマトグラフィーまたミリポアフィルターによるbinding assayで調べた。その結果, ダイズまた糸状菌いずれの場合もパルスラベルした全RNAの約1%がpoly (A)-containing messenger RNAとして存在することが明らかとなった。ショ糖密度勾配遠心法によると, それらのpoly (A)-containing messenger RNAは非常に大きい分子量をもつと思われるピークを除いては, そのサイズがneterogenousであった。また, P. megasperma var. sojaeの培養ろ液中にはダイズ胚軸におけるpoly (A)-containing messenger RNAの合成を促進する活性が認められた。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"^3H-Uridine pulse-labeled RNA isolated from soybean hypocotyl and the two plant pathogenic fungi, Phytophthora megasperma var. sojae and P. capsici was analysed by an affinity chromatography on Sepharose 4B-poly U column and by Millipore binding assay for poly (A)-containing messenger RNA. Poly (A)-containing messenger RNA amounted to as much as 1% of total pulse-labeled RNA from either soybean or the two fungi. Sucrose density gradient centrifugation revealed that poly (A)-containing messenger RNA from the three sources was heterogenous in size except one which possessed a prominent peak at the bottom of the gradient. A culture filtrate of P. megasperma var. sojae contained substance (s) that stimulated the synthesis of poly (A)-containing messenger RNA by soybean hypocotyl to approximately 2-folds of the non-treated control.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062275","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00757373","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"吉川, 正明","creatorNameLang":"ja"},{"creatorName":"ヨシカワ, マサアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Yoshikawa, Masaaki","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"正子, 朔","creatorNameLang":"ja"},{"creatorName":"マサゴ, ハジメ","creatorNameLang":"ja-Kana"},{"creatorName":"Masago, Hajime","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000078964.pdf","filesize":[{"value":"433.0 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000078964.pdf","url":"https://kpu.repo.nii.ac.jp/record/4965/files/KJ00000078964.pdf"},"version_id":"d9ba1706-256e-4126-8cda-1d124b08302a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Presence of poly (A)-containing messenger RNA in soybean and Phytophthora spp (Agriculture)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Presence of poly (A)-containing messenger RNA in soybean and Phytophthora spp (Agriculture)","subitem_title_language":"en"}]},"item_type_id":"3","owner":"14","path":["420"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"4965","relation_version_is_last":true,"title":["Presence of poly (A)-containing messenger RNA in soybean and Phytophthora spp (Agriculture)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-04-24T05:32:27.012538+00:00"}