{"created":"2023-06-19T08:46:12.470719+00:00","id":4964,"links":{},"metadata":{"_buckets":{"deposit":"edb6b35b-51fa-4e37-82e3-dfe73f0d4749"},"_deposit":{"created_by":14,"id":"4964","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"4964"},"status":"published"},"_oai":{"id":"oai:kpu.repo.nii.ac.jp:00004964","sets":["47:273:420"]},"author_link":["8401"],"control_number":"4964","item_1696926521561":{"attribute_name":"その他（別言語等）","attribute_value_mlt":[{"subitem_alternative_title":"グラジオラス球茎の休眠打破におけるサイトカイニンの作用機構(農学部門)","subitem_alternative_title_language":"ja"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1976-11-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"50","bibliographicPageStart":"40","bibliographicVolumeNumber":"28","bibliographic_titles":[{"bibliographic_title":"京都府立大學學術報告. 農學","bibliographic_titleLang":"ja"},{"bibliographic_title":"The scientific reports of Kyoto Prefectural University. Agriculture","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"サイトカイニン類が種々の球根植物の休眼打破に効果があることは, 塚本らがすでに報告している。本報告では, グラジオラス球茎の休眠覚醒にともなう内生サイトカイニンの消長とその作用機構を検討した。一般に認められているグラジオラス球茎の休眠打破に必要な低温処理を行っても, 処理直後では内生サイトカイニンの活性化はほとんど認められなかったが, 低温処理後10日間高温(30℃)下においた球茎では内生サイトカイニン活性の著しい増加が認められた。種々の条件で貯蔵した球茎の頂芽部(0.2(cm)^3)を無菌培養し, その発芽状態を調べたところ, 低温処理直後の球茎の頂芽部は発芽しないが, 低温処理後30℃に2週間貯蔵した球茎の頂芽部は培養3週間でほぼ100%の発芽率が認められた。一方低温処理直後の球茎の頂芽部を1ppmベンジルアデニンを含む培地で培養するとすべての個体が発芽した。頂芽部の培養組織片の大きさを大(1(cm)^3), 中(0.5(cm)^3), 小(0.2(cm)^3)に分けて培養したところ, 大きい組織片では低温処理直後に培養しても発芽するが, 中および小さい組織片では低温処理後10&acd;15日間30℃に貯蔵した球茎でないと発芽しなかった。また, 収穫直後の球茎から大, 中, 小の頂芽部組織片を切りとり, 試験管内で5週間低温処理を行い, 以後25℃で45日間培養しても発芽する個体は認められなかった。つぎに, ベンジルアデニン-8-^<14>Cを用い, サイトカイニンとグラジオラス球茎の発芽抑制物質(アブシジン酸, ピロガロール, クマリン)の組合せ処理による, サイトカイニンの球茎の休眠打破における作用機作を検討した。その結果, 抑制物質と^<14>C-ベンジルアデニン混合処理区での^<14>CのRNAへのとりこみ率が^<14>C-ベンジルアデニン単独処理区にくらべて非常に低かった。このことから抑制物質とサイトカイニンがRNA代謝に関与して, グラジオラス球茎の休眠を制御しているものと思われる。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"As reported in our previous papers, cytokinin is effective in breaking dormancy of gladiolus corms, and hence its action mechanism was investigated in the present study. Changes in the level of endogenous cytokinin activity in the corms in the releasing process from dormancy caused by low temperature was examined. The activity was hardly detectable in the corms immediately after low temperature storage for 5 weeks, in spite that this treatment was sufficiently effective on breaking dormancy. When the corms were further stored at high temperature for 10 days, however, cytokinin activity in the corms rapidly increased. It seems possible to postulate from these results, that the process of cytokinin synthesis in the corms is prepared by exposure to low temperature and the start of the actual cytokinin synthesis is triggered by high temperature following exposure to low temperature. In the experiment of the in vitro culture of the buds isolated from the corms stored under various conditions, cytokinin synthesis was recognized after low temperature treatment followed by high temperature. The size of explants was related to the ability to sprout, so that it seems to have certain bearing on the process of cytokinin synthesis. In the experiment using ^<14>C-BA, inhibitors (abscisic acid, pyrogallol and coumarin) which antagonize cytokinin on sprouting of the corms, suppressed the incorporation of cytokinin into RNA. These results indicate that the antagonism between cytokinin and inhibitors in breaking dormancy is related to RNA metabolism.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00062275","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00757373","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"矢澤, 進","creatorNameLang":"ja"},{"creatorName":"ヤザワ, ススム","creatorNameLang":"ja-Kana"},{"creatorName":"Yazawa, Susumu","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"KJ00000078963.pdf","filesize":[{"value":"915.9 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"KJ00000078963.pdf","url":"https://kpu.repo.nii.ac.jp/record/4964/files/KJ00000078963.pdf"},"version_id":"b94bb5af-12ce-4e8f-a86b-034c23af1526"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"The action mechanism of cytokinin in breaking dormancy of gladiolus corm (Agriculture)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The action mechanism of cytokinin in breaking dormancy of gladiolus corm (Agriculture)","subitem_title_language":"en"}]},"item_type_id":"3","owner":"14","path":["420"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-20"},"publish_date":"2017-02-20","publish_status":"0","recid":"4964","relation_version_is_last":true,"title":["The action mechanism of cytokinin in breaking dormancy of gladiolus corm (Agriculture)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-03-21T02:13:49.363789+00:00"}