@article{oai:kpu.repo.nii.ac.jp:00004954,
 author = {伊吹, 文男 and Ibuki, Fumio and 田中, 春代 and Tanaka, Haruyo and 三好, 正満 and Miyoshi, Masamitsu and 金森, 正雄 and Kanamori, Masao},
 journal = {京都府立大學學術報告. 農學, The scientific reports of Kyoto Prefectural University. Agriculture},
 month = {Nov},
 note = {牛κ-カゼインの不均一性を調べるため, Zittle-Custer法で調製したκ-カゼインを, β-メルカプトエタノールで還元した後, 0.02Mイミダゾール・塩酸緩衝液(pH 7.0), NaCl濃度勾配(0.02M∿0.2M)を用いて, DEAEセルロースクロマトグラフィーをおこない, 非吸着画分を含めて6画分を得た。(P-1∿P-6)澱粉ゲル電気泳動(pH 8.6)において, P-1は負極へ移動するが, 他の5画分は, 高い塩濃度で溶出する画分ほど, 正極へより多く移動する傾向が見られた。6画分の化学組成を調べた結果, シアル酸含有量に著しい差異が見られ, P-1とP-2には全くシアル酸が含まれず, P-3∿P-6では, 高い塩濃度で溶出する画分ほど, シアル酸を多く含んでいた。アミノ酸組成は, どの画分もほぼ同じであった。6画分のうち, P-1以外は, κ-カゼインの特徴であるα_S-カゼイン安定化能を有している。, The heterogeneity of bovine κ-casein was studied. κ-Casein was prepared from fresh bovine milk by the method of Zittle and Custer. After the reduction of κ-casein with β-mercaptoethanol, it was fractionated by chromatography on a column of DEAE cellulose with linear gradient system of sodium chloride (0.02M to 0.2M) in imidazole-HCl buffer, pH 7.0. Six fractions (P-1 to P-6) were obtained including a non absorbed fraction to the column. In the starch gel electrophoresis, the fractions migrated to anode except the fraction P-1 which was mobile to the cathode. The sequence of mobilities in electrophoresis coincides exactly with the sequence of the elution on DEAE cellulose chromatography in the gradient system. The amino acid compositions of these fractions are almost the same except the fraction P-1 but the sialic acid contents are different greatly. All these fractions (P-2 to P-6) had the ability of stabilizing α_S-casein and the removement of sialic acid is of no effect on that stabilizing effect. It is concluded that the heterogeneity of κ-casein is attributed to the difference of the sialic acid contents.},
 pages = {110--115},
 title = {牛κ-カゼインの不均一性に関する研究(農芸化学部門)},
 volume = {27},
 year = {1975},
 yomi = {イブキ, フミオ and タナカ, ハルヨ and ミヨシ, マサミツ and カナモリ, マサオ}
}