@article{oai:kpu.repo.nii.ac.jp:00004824,
 author = {広瀬, 忠彦 and Hirose, Tadahiko and 玉井, 道敏 and Tamai, Michitoshi and 土肥, 尚 and Dohi, Takashi and 高嶋, 四郎 and Takashima, Shiro},
 journal = {京都府立大學學術報告. 農學, The scientific reports of Kyoto Prefectural University. Agriculture},
 month = {Oct},
 note = {ナス科果菜類のF_1採種の能率化をさらに進めるためにはそれらの生殖に関する生理学的究明が基礎的に重要である。その研究の一環として, まずナス花粉の発芽生理を明らかにするために人工発芽床に種々の物質を添加してそれらの影響を調べた。7cm径のプラスチック製ペトリざらを用い, ショ糖17%, 1級寒天1%, pH5.5の基本培地に各種の被験物質を添加して花粉をまき, 30℃にて6時間おいたのちに固定して鏡検測定した。その結果はつぎのとおりである。NAA, IAA, CCCおよびB-995の添加は花粉の発芽率を高めるが, GA, HMCPAならびにMHの添加では花粉の発芽および管伸長のいずれをも促進しない。核酸塩基類の添加も花粉発芽にはほとんど影響を及ぼさない。アミノ酸類ではプロリンとシステインの添加が発芽促進効果を示す。無機塩類を添加すると多くのものが発芽を阻害するが, カリウムはごくわずか, ホウ素はきわめて顕著に発芽伸長を促進する。ホウ酸を用いた場合は10ppmのホウ素濃度で管伸長速度が毎時375μに達する。これは花柱切除法による生体内での伸長速度の測定結果とほぼ一致する。以上の結果から, ナス花粉の発芽および管伸長には糖以外ではホウ素の存在が決定的に重要な役割を果たしており, 他の諸物質の必要性はあるとしても補助的なものと考えられる。, In order to improve on the hybrid seed production in several Solanaceous vegetables, i.e. tomato, pepper and eggplant, physiological and anatomical studies of their reproduction are fundamental. For the first step in these studies physiological research on the germination of eggplant (Solanum Melongena L.) pollen was done. All of the experiments in the present study were carried out using an artificial media which contained seventeen per cent sucrose, one per cent purified agar and certain substances to be tested at different concentrations. The pH value of the media was adjusted at 5.5. Fresh pollen was sown on the media solidified in the plastic Petri dishes, and incubated at 30℃. After six hours they were fixed with cotton blue-lactic phenol solution and observed. The results and conclusions obtained from the study are as follows. 1) The germination rate of eggplant pollen was slightly increased by the supply of alpha-naphtha-leneacetic acid, beta-indoleacetic acid, 2-chloroethyl trimethylammonium chloride and N-dimetyl amino succinamic acid, while no promotional effects on pollen germination or tube growth were observed after supplying gibberellic acid, 2-hydroxymethyl 4-chlorophenoxyacetic acid, maleic hydrazide, adenin, uracil or hypoxanthine. The supply of free amino acids with the exception of proline and cysteine had no promotional effects on pollen germination. 2) Most of the inorganic salts inhibited both pollen germination and tube growth. Boron, on the contrary, promoted them markedly in all concentrations examined. On the medium containing 57.13ppm of boric acid (10ppm of boron) the pollen tubes grew at the rate of 375 microns an hour. This rate of growth agreed approximately with their natural growth rate in the pistil. Calcium and magnesium ions, both inhibited pollen germination and tube growth by themselves. However, when either calcium or magnesium was used to supplement boron, pollen germination and tube growth were promoted at a rate somewhat greater than when boron was used alone. 3) From the results obtained in the present study, it may be concluded that in addition to sugar boron plays an essential role in the germination and tube growth of eggplant pollen. The other substances are subsidiary even if they are necessary.},
 pages = {25--31},
 title = {ナス科果菜類の生殖に関する研究 I : ナス花粉の発芽に対する諸物質の影響(農学部門)},
 volume = {20},
 year = {1968},
 yomi = {ヒロセ, タダヒコ and タマイ, ミチトシ and ドヒ, タカシ and タカシマ, シロウ}
}