@article{oai:kpu.repo.nii.ac.jp:00004682, author = {竹岡, 政治 and TAKEOKA, MASAJI}, journal = {京都府立大學學術報告. 農學, The scientific reports of Kyoto Prefectural University. Agriculture}, month = {Sep}, note = {この報告は, 花粉膜の表面微細構造によるツガ及びカナダツガの類別について述べるものである。供試料は, Micropeuce (Eutsuga)に属するツガ及びカナダツガの2種である。実験方法は, 筆者が1956年来適用しているMethylmethacrylate carbon 2段replica法によつた。実験の結果, 下記の諸点を明らかにすることができた。1.ツガ花粉 : cap及びgerm furrowの表面は不規則に配列した隆状突起によつておおわれ, さらにそれより大きさの異なつた指状物が突出し, その間にごく小数ながらイボ状物の存在が認められる。とくに指状突起物は軟弱なためか, 曲りやすく, しかもほとんどはく離しない。一方, marginal ridgeとgerm furrowの連結部の膜面は, 微小なしかも不規則な突起物によつておおわれている。これはcap及びgerm furrowの表面と全く異なつた構造を呈している。また, Photo. 3に示した如く, germ furrowの中央部にnarbeの存在を確認することができた。narbeの表面は, へいかつである。指状突起物の長さは約0.3∿2.1μ, 連結部膜面の突起物の長さは約0.1∿0.2μ, またnarbeは長径約7.5μである。2.カナダツガ花粉 : capの表面は花野菜型の隆起でおおわれ, その表面には微小な刺状突起をそなえている。またgerm furrowの表面には, 大きさの異なつた粒状隆起が存在し, さらにその表面には微小な刺状紋を有しており, 粒状隆起の間には不定形の隆起が認められた。capとgerm furrowの連結部の模面構造はツガ花粉と同様である。Photo. 6に示したように, ツガにみられたnarbeが見出しえられなかつた花野菜型隆起部上の刺状紋の長さは約0.1μであり, 粒状突起物の直径は約2.4∿2.0μで, またその表面に存在する刺状紋の長さは約0.1μである。以上述べた諸点で明らかなように, ツガ及びカナダツガ花粉の微細構造は, いちじるしく異なつている。なおPhoto. 7に示したイボ状構造は, ectosexineより下の層の表面構造である。筆者は, ツガ花粉のsexineは3つの異なつた表面構造をもつ層から成り立つているのであろうということを推定した。, The present paper deals with the classification between Tsuga Sieboldiana and Tsuga canadensis, based on the fine surface structure of pollen membrane. The matrials used in this study are the pollen of 2 species, such as Tsuga Sieboldii CARR. and Tsuga canadensis CARR. belonging to Micropeuce (Eutsuga). The experimental method depends upon the methylmethacrylate-carbon 2 stage replica, which was used by the author since 1956. As the result of the electronmicroscopic investigation, he could clarify the following points. 1. Pollen of Tsuga Sieboldii CARR. : the surface of cap and germ furrow is covered all over with irregularly arranged protrusions, and finger-like projections which are different in sizes, rising from their surface. Especially, their projections are pliant, probably on account of their delicate nature, and they cannot, in most cases, be separated from the surface of the grain. Besides, the surface of the combined parts between marginal ridge and germ furrow of the grain is covered with fine and irregular projections. As shown in Photo 1,we can seen the different structure of the surface pattern of cap, and germ furrow. As given in photo. 3,he could recognise the existence of narbe in the central part of germ furrow. The surface of narbe is very smooth. The length of finger-like projection; about 0.3∿2.1μ, the length of projection of surface membrane in the combined parts; about 0.1∿0.2μ, and long diameter of narbe; about 7.5μ. 2. Pollen of Tsuga canadensis CARR. : the surface of cap is covered all over with protrusions of the "cauli-flower type", and they are possessed of projection of fine spinules on its surface. Also, on the surface of germ furrow were seen grain-like projections in different sizes, and fine spinules on its surface. Moreover, protrusion of amorphus in that of grain-like projection were recognised. The structure of the combined part of cap and germ furrow are similar to those of Tsuga Sieboldii pollen. As given in photo. 6,there is no find of narbe as are found in Tsuga Sieboldii pollen. The length of spinules (on protrusions of the "cauli-flower type"); about 0.1μ, diameter of grain-like projection; about 0.4∿2.0μ, and the length of the spinules existed in their surface; about 0.1μ. As mentioned above, it is clearly seen that the fine structure between the pollen of Tsuga Sieboldii and of Tsuga canadensis were considerably different. Moreover, the verrucate structure as given in photo. 7 shows the surface structure of the under layer of ectosxine in sexine stratification. It has been estimated that sexine of Tsuga Sieboldii pollen consist of three layers each different in the fine surface structure.}, pages = {83--"86-3"}, title = {花粉膜の表面微細構造によるツガ及びカナダツガの類別について(林学部門)}, volume = {12}, year = {1960}, yomi = {タケオカ, マサジ} }