@article{oai:kpu.repo.nii.ac.jp:00004662, author = {今原, 広次 and Imahara, Hirotsugu}, journal = {京都府立大學學術報告. 農學, The scientific reports of Kyoto Prefectural University. Agriculture}, month = {Sep}, note = {1糖蜜を使用せる培地に於てEndomyces (FH)菌を培養する事に依り次の事実を認めた。a.エタノールを添加するとFH菌の生成菌体量及び生成油脂量が増大する又エタノールの添加は二回に分けて行う方が僅かに良好な結果を得た。b.エタノール添加の代りにFH菌を酵母と共棲醗酵せしめる場合も同様な効果を得た。c.ビタミンを添加するとビタミンB_1及びニコチン酸添加の場合のみ生成菌体量及び生成油脂量の増大を認め得たが, ビタミンB_2パントテン酸石灰及びビタミンB_6は良い結果を与えなかつた。又ビタミンの添加量は10mg/100cc程度で充分であつた。2合成培地の炭素源として夫々葡萄糖及びエタノールを使用した場合のビタミン添加の影響を検討した所, 葡萄糖の場合はビタミンB_1及びニコチン酸が良好な結果を与えたが, アルコールの場合はビタミンB_1のみが良好な結果を与えた。3糖蜜を基質とした場合の菌体内油脂含量は31∿34%位であり, 葡萄糖基質の場合は17%前後, エタノール基質の場合は14%前後で, この油脂含有率はビタミンの添加によつて著しい変化が起る事は認められずこの際の油脂収量の増大は生成菌体が増大する事に基く事を認めた。本研究は本学当講座主任, 中浜敏雄教授の御指導に依るもので, ここに深謝の意を表する。, 1. The fat formation of Endomyces species, FH, was studied when it was cultured in the molasse medium. On the result of addition of ethanol to the medium, the amount of total fat produced by FH was increased according to the increasing of the pellicle. By symbiosis of FH with Saccharomyces sake, the similar effect as the addition of ethanol was shown. When I added vitamin B_1 or nicotinic acid to the culture solution, the quantity of the pellicle and total fat produced by FH were both increased and I could not show the similar effect by the addition of the other vitamines which were experimented. 2. The influence of addition of vitamines was examined in the synthetic medium which was used glucose or ethanol as the carbon source. Both vitamin B_1 and nicotinic acid were effective in glucose medium, while only vitamin B_1 was effective in the ethanol medium. 3. The fat contents of the pellicle was 31-34% on the molasse medium, about 17% on the glucose medium and about 14% on the ethanol medium. On each case, the fat content in the cell should not be shown remarkable change by the addition of vitamines and the increasing of yield in the certain culture solution due to the increasing of the pellicle quantity.}, pages = {164--168}, title = {微生物の油脂生成(第 5 報) : 油脂生成機能に対するビタミンの影響}, volume = {11}, year = {1959}, yomi = {イマハラ, ヒロツグ} }