@article{oai:kpu.repo.nii.ac.jp:00004638, author = {村上, 道夫 and 竹岡, 政治 and MURAKAMI, MICHIO and TAKEOKA, MASAJI}, journal = {京都府立大學學術報告. 農學, The scientific reports of Kyoto Prefectural University. Agriculture}, month = {Sep}, note = {筆者らは現在改良試験を実施中であるCoix属のハトムギ, ジュズダマ及びそのF_1雑種, 更にその四倍体植物の花粉を, Zea属のトウモロコシ花粉と共に薬品処理による花粉膜の光学顕微鏡的観察と, Carbon Replica法の適用による花粉膜の表面微細構造の電子顕微鏡的観察を行い, 両者の比較を行つた。1.光学顕微鏡的観察 光顕像においては, 供試植物の花粉の間には, 花粉粒の大きさ以外に形態学的差異は観察されなかつた。発芽孔は一つであり, 花粉膜はextineが薄くintineが厚く観察された。また発芽帯周辺は一般に厚膜であり, 花粉の変形, 壌裂は極の方に多い。発芽帯には乳状突起が観察された。2.電子顕微鏡的観察 電顕像による花粉のextexineの微細構造は, 供試花粉ではいづれも微細なspineによつておおわれ, 各種間に明らかな差は認められない。ただCoix, Zea両属間にはspineの大きさにおいて, Coix属内の各種間には形状においてやや差が認められるようである。発芽帯の状態は, トウモロコシでは乳状突起の中心に, spineをもつ隆起部が発達し, ハトムギ及びジュズダマでは共にこの隆起部はなく, わづかなうねりをもち, なお乳状突起には不規則に少数のspineが点在していた。しかし, このspineをもつ隆起部は, F_1及び四倍体植物においては著しく発達していた。発芽帯の孔径は, 四倍体は二倍体に比して著しく大きかつた。一般にCoix属とZea属の花粉表面構造の間には, きわめて高度の類似性が認められた。, The morphology of pollen is investigated as a main section of palynology. In the present time the studies on the surface structure of pollen are paid special attention to make progress of the pollen analysis. The present paper deals with the results of microscopic and electromicroscopic investigations on the surface structure of the pollen membrane. The materials used in this studies are the pollen of Zea mays, Coix Ma-Yuen, C. Lacryma-Jobi, F_1 plant (C. Ma-Yuen×C. Lacryma-Jobi), 4x F_1 plant and 4x C. Ma-Yuen. The pollen used in the microscopic observation was treated by HCI and NaOH previously and electromicroscopic observation was based on the carbon replica method. 1. Microscopic observation : The morphological difference of pollens between the material plants was not recognized except the diameter of these pollen grains. The pollen of Zea and Coix plant are apaturate pollen similarly other gramineae plants. The extine of pollen membrane was thin but the intine was observed very thickly, These pollens have thick membrane around a germinal zone, for that reason the transformation and the bursting of pollen occurs near the pole of pollen, In germ pore of pollen grain the papilla was observed slightly. 2. Electromicroscopic observation : According to electromicroscopic observation, the extexine of pollen membrane was covered with fine spinules, there were no significant differences between the material plants on the surface structure of the pollen membrane. It is, however, obviously to the slight difference were recognized in the size of spine between the genus Coix and Zea, and in the shape of spine between the each plants in genus Coix. In the state of germ pore, there are fairly different between the material plants. In the germ pore of Zea pollen, the protrusion with fine spinules was well-developed in the central part of papilla, on the contrary, in the Coix Ma-Yuen and C. Lacryma-Jobi a few spines are dotted irregularly on the whole surface of slight concaved papilla. The protrusion with fine spinules was not found in the other pollen of C. Ma-Yuen and C. Lacryma-Jobi. But the protrusion was well-developed in the pollen of F_1 plant, 4x F_1 plant and 4x C. Ma-Yuen. The diameter of germ pore of 4x Coix pollen was larger as compared with that of 2x Coix pollen. As the results of these experiment it wes revealed generally that the similarity between the surface structure of pollen membrane of genus Coix and Zea was very closely.}, pages = {9--"14-3"}, title = {Coix 属の改良に関する育種学的研究 (III) : Coix 属及び Zea 属の花粉膜の表面構造について}, volume = {11}, year = {1959}, yomi = {ムラカミ, ミチオ and タケオカ, マサジ} }