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Presence of poly (A)-containing messenger RNA in soybean and Phytophthora spp (Agriculture)
https://kpu.repo.nii.ac.jp/records/4965
https://kpu.repo.nii.ac.jp/records/49658a135881-6d2e-4267-9db0-2ab302374ae9
名前 / ファイル | ライセンス | アクション |
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KJ00000078964.pdf (433.0 kB)
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Item type | [ELS]紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-02-20 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Presence of poly (A)-containing messenger RNA in soybean and Phytophthora spp (Agriculture) | |||||
その他(別言語等) | ||||||
その他のタイトル | ダイズ植物ならびにその病原菌 Phytophthora spp. における poly (A)-containing messenger RNA の存在(農学部門) | |||||
言語 | ja | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
吉川, 正明
× 吉川, 正明× 正子, 朔 |
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抄録(日) | ||||||
内容記述タイプ | Other | |||||
内容記述 | ダイズ胚軸および2種の植物病原菌, Phytophthora megasperma var. sojaeとP. capsiciを^3H-uridineでパルスラベルし, それらから得たRNA中にpoly (A)-containing messenger RNAが存在するかどうかについて, セファロース4B-poly Uカラムによるaffinityクロマトグラフィーまたミリポアフィルターによるbinding assayで調べた。その結果, ダイズまた糸状菌いずれの場合もパルスラベルした全RNAの約1%がpoly (A)-containing messenger RNAとして存在することが明らかとなった。ショ糖密度勾配遠心法によると, それらのpoly (A)-containing messenger RNAは非常に大きい分子量をもつと思われるピークを除いては, そのサイズがneterogenousであった。また, P. megasperma var. sojaeの培養ろ液中にはダイズ胚軸におけるpoly (A)-containing messenger RNAの合成を促進する活性が認められた。 | |||||
言語 | ja | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | ^3H-Uridine pulse-labeled RNA isolated from soybean hypocotyl and the two plant pathogenic fungi, Phytophthora megasperma var. sojae and P. capsici was analysed by an affinity chromatography on Sepharose 4B-poly U column and by Millipore binding assay for poly (A)-containing messenger RNA. Poly (A)-containing messenger RNA amounted to as much as 1% of total pulse-labeled RNA from either soybean or the two fungi. Sucrose density gradient centrifugation revealed that poly (A)-containing messenger RNA from the three sources was heterogenous in size except one which possessed a prominent peak at the bottom of the gradient. A culture filtrate of P. megasperma var. sojae contained substance (s) that stimulated the synthesis of poly (A)-containing messenger RNA by soybean hypocotyl to approximately 2-folds of the non-treated control. | |||||
言語 | en | |||||
書誌情報 |
ja : 京都府立大學學術報告. 農學 en : The scientific reports of Kyoto Prefectural University. Agriculture 巻 28, p. 51-56, 発行日 1976-11-30 |
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雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00062275 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 00757373 |