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電気的形質転換法を用いた cDNA ライブラリーの作製(農芸化学部門)
https://kpu.repo.nii.ac.jp/records/5175
https://kpu.repo.nii.ac.jp/records/5175ec51cf3b-63f3-4b09-a7b1-ed9f377f3420
名前 / ファイル | ライセンス | アクション |
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KJ00000079174.pdf (400.6 kB)
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Item type | [ELS]紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-02-20 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 電気的形質転換法を用いた cDNA ライブラリーの作製(農芸化学部門) | |||||
その他(別言語等) | ||||||
その他のタイトル | Construction of cDNA library using electro-transformation(Agricultural Chemistry) | |||||
言語 | en | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
田中, 國介
× 田中, 國介× 若浦, 誠× 増村, 威宏× 藤井, 昭治 |
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抄録(日) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 電気的形質転換法を用いプラスミドDNAを導入することにより大腸菌を形質転換した。我々は高効率を得る工夫をし, この方法を用いることでコンピテントセルを用いる形質転換法(Hanahan法)では出せない高効率を得ることが可能になった。ここではこの方法で登熟期イネ胚乳及びホウレンソウ芽生えよりcDNAライブラリーの作製に成功した。 | |||||
言語 | ja | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | To transform Escherichia coli a plasmid DNA was introduced by electroporation. We improved transformation efficiency adding several chemicals, biological materials and/or preheating of the buffer solution which was added just affer electrodischarge. The improved method gave higher transformation efficiency comparing with that of Hanahan's. cDNA libraries for mRNAs appearing in the developing rice seeds and spinach seedlings were constructed using this method. | |||||
言語 | en | |||||
書誌情報 |
ja : 京都府立大學學術報告. 農學 en : The scientific reports of Kyoto Prefectural University. Agriculture 巻 41, p. 86-92, 発行日 1989-11-18 |
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雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00062275 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 00757373 |